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. 2021 Jun 3;12:3320. doi: 10.1038/s41467-021-23601-2

Fig. 1. PLIN5 expression in brown adipose tissue is regulated by temperature and generation and validation of inducible, BAT-specific PLIN5 transgenic mice (BATiPLIN5).

Fig. 1

a Timeline for controlled temperature experiments depicted in b and c. We collected samples for RNA and western blot (WB) at the indicated time points. b qPCR for Plin5 relative mRNA expression in brown adipose tissue (BAT) from wild-type mice (C56BL/6J) housed at the indicated temperatures. n = 3 mice per group. c WB for PLIN5 in BAT from wild-type mice (C56BL/6 J) housed at the indicated temperatures. d Timeline for controlled temperature experiment depicted in e. e BAT WB for PLIN5 from wild-type C56BL/6 J mice housed at the indicated temperatures (left panel) and WB quantification of PLIN5 normalized to GDI (right panel). n = 2 mice per group. f Schematic representation for doxycycline-inducible myc-PLIN5 overexpression mice in BAT (BATiPLIN5 mice). g qPCR for Plin5 relative mRNA expression from the indicated tissues from Control or BATiPLIN5 mice housed at 23 °C. n = 3 mice per group. h WB for PLIN5 from BAT of Control (n = 8 mice) or BATiPLIN5 (n = 9 mice) housed at 23 °C (left panel). WB quantification of PLIN5 normalized to GAPDH (right panel). Values are mean ± s.e.m. For b, statistical analysis was performed using one-way ANOVA followed by Tukey posttest. For g and h, statistical analysis was performed using unpaired two-sided Student’s t test. Source data are provided as a Source data file.