Table 2.
Primer sequences.
| Gene | Forward | Reverse | Use |
|---|---|---|---|
| Plin5 | GAGGCAGCAACAGGGCTACT | CAAAGAGTGTTCATAGGCGAGATG | qPCR |
| 18S | GAG CGA AAG CAT TTG CCA AG | GGC ATC GTT TAT GGT CGG AA | qPCR |
| Ucp1 | CCC TGG CAA AAA CAG AAG GA | AGC TGA TTT GCC TCT GAA TGC | qPCR |
| Elov3 | GCC AAA CTG AAG CAT CCT AAT CTT | CCC AGA ACC ATC TGC AGA ATC | qPCR |
| Ppargc1a | TGC CAT TGT TAA GAC CGA G | TTG GGG TCA TTT GGT GAC | qPCR |
| Dio | AAG AAG CAC CGG AAC CAA GA | GGC GGC AAG GAG AAA CG | qPCR |
| Cidea | GGA GAC CGC CAG GGA CTA C | TTA GTC TGC AAT CCC ATG AAT GTC | qPCR |
| Prdm16 | TCC GCG GTC AGC AAT AGC | TCT TCC AAG CGG CCA TCA | qPCR |
| Tfam | CCAAAAAGACCTCGTTCAGC | ATGTCTCCGGATCGTTTCAC | qPCR |
| Slc2a4 (Glut4) | GCT TTG TGG CCT TCT TTG AGA | CTG AAG AGC TCT GCC ACA ATG A | qPCR |
| Fgf21 | GCT GCT GGA GGA CGG TTA CA | CAC AGG TCC CCA GGA TGT TG | qPCR |
| Cd36 | GGA GGC ATT CTC ATG CCA GT | CTG CTG TTC TTT GCC ACG TC | qPCR |
| Lpl | CCA GCT GGG CCT AAC TTT GA | AAC TCA GGC AGA GCC CTT TC | qPCR |
| Angptl4 | GTT TGC AGA CTC AGC TCA AGG | CCA AGA GGT CTA TCT GGC TCT G | qPCR |
| Slc27a1 (Fatp1) | TCC TAA GGC TGC CAT TGT GG | GCA CGC ATG CTG TAG GAA TG | qPCR |
| Il6 | CCA GAG ATA CAA AGA AAT GAT GG | ACT CCA GAA GAC CAG AGG AAA T | qPCR |
| Adgre1 (F4/80) | CTT TGG CTA TGG GCT TCC AGT C | GCA AGG AGG ACA GAG TTT ATC GTG | qPCR |
| Saa3 | CCT GGG CTG CTG CTA AAG TCA TC | ACC CAG TAG TTG CCC CTC TT | qPCR |
| Tnfa | GAG AAA GTC AAC CTC CTC TCT G | GAA GAC TCC TCC CAG GTA TAT G | qPCR |
| Ccl2 (Mcp1) | AGC ACC AGC CAA CTC TCA C | TCT GGA CCC ATT CCT TCT TG | qPCR |
| Rsp18 | TGT GTT AGG GGA CTG GTG GAC A | CAT CAC CCA CTT ACC CCC AAA A | qPCR (mtDNA)74 |
| Cox2 | ATA ACC GAG TCG TTC TGC CAA T | TTT CAG AGC ATT GGC CAT AGA A | qPCR (mtDNA)74 |
| Acss2 | GCT TCT TTC CCA TTC TTC GGT | CCC GGA CTC ATT CAG GAT TG | qPCR41 |
| Acaca | GGA GAT GTA CGC TGA CCG AGA A | ACC CGA CGC ATG GTT TTC A | qPCR41 |
| Fasn | GCT GCG GAA ACT TCA GGA AAT | AGA GAC GTG TCA CTC CTG GAC TT | qPCR41 |
| Chrebpα | CGA CAC TCA CCC ACC TCT TC | TTG TTC AGC CGG ATC TTG TC | qPCR41 |
| Chrebpβ | TCT GCA GAT CGC GTG GAG | CTT GTC CCG GCA TAG CAA C | qPCR41 |
| Dgat2 | AGT GGC AAT GCT ATC ATC ATC GT | TCT TCT GGA CCC ATC GGC CCC AGG A | qPCR |
| Th | GAA GGG CCT CTA TGC TAC CCA | TGG GCG CTG GAT ACG AGA | qPCR |
| Dbh | GAC TCA ACT ACT GCC GGC ACG T | CTG GGT GCA CTT GTC TGT GCA GT | qPCR |