Figure 5.

PAC promotes autophagy in oral cancer cells by targeting LC3B and p62 protein expression. (A) in vitro effect of PAC on Ca9-22 cells autophagy: Ca9-22 cells were stimulated or not with various concentrations of PAC for 24 h, then incubated with a diluted Autophagy Probe, Red at 1:50 for 1 h in the dark. Stained cells were afterwards analyzed by flow cytometry, (n = 3). (B) Statistics analysis of fold changes in % of staining autophagy cells between ca9-22 treated by PAC concentration and Ca9-22 untreated cells (t = test, p < 0.005; n = 3). (C) Western Blot showing an overexpression at protein levels of two autophagy markers (LC3B and p62) in Ca9-22 cells treated by 5 µM of PAC (n = 3). All Blots for each autophagy protein derive from the same experiment and were exposed at same time of phosphor imager exposition.