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. 2021 May 21;8:660246. doi: 10.3389/fcvm.2021.660246

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Copyright © 2021 Gu, Chen, Jin, Liu, Xu, Liu, Luo, Ling, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of different constrictions on myocardial hypertrophy and angiogenesis. (A) Immunostaining for CD31 (red), wheat germ agglutinin (WGA, green), and DAPI in heart sections. Scale bar: 20 μm. (B) Quantification of CD31⁺ capillaries/field; n = 3 hearts per group. Each heart slide was random selected with five high-power fields for statistical analysis. (C) Analysis of cardiomyocyte cross-sectional area in the three groups; n = 3 hearts per group. A total of 300 cardiomyocytes per group were analyzed. (D,E) Immunostaining of heart slides for CD31 and quantification of capillary density. Scale bar: 20 μm; n = 3 hearts per group. Each heart slide was random selected with five to eight high-power fields for statistical analysis. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001, and ****P < 0.0001. Statistical significance was calculated using the unpaired two-tailed t-test.