. 2021 May 21;8:654125. doi: 10.3389/fvets.2021.654125

Table 3.

Phenotypic resistance and presence of associated antimicrobial resistance genes for the most multidrug resistant Escherichia coli (n = 8) isolated from calf of 101 dairy farms in Québec, Canada.

Antimicrobial		Isolate
Class	Agent	10040013	10420013	10500013	10600013	10780027	10830027	10940027	10990027
Aminocyclitol	SPT	aadA1	aadA1, aadA2	aadA1, aadA2	aadA1	aadA2	aadA2	aadA1	aadA5
Aminoglycoside	GEN					aac(3)-IId			aac(3)-IId
	NEO				aph(3')-Ia	aph(3')-Ia	aph(3')-Ia		aph(3')-Ia
	STR	aadA1 strA/strB	aadA1, aadA2	aadA1, aadA2 strA/strB	aadA1 strA/strB	aadA2 strA/strB	aadA2 strA/strB	aadA1 strA/strB	aadA5 strA/strB
Aminopenicillin	AMP	bla_CMY−2, bla_TEM−1B	bla_CARB−2	ampC^*	bla_CMY−2 bla_TEM−1B	bla_CMY−2 bla_TEM−1B	ampC^*	ampC^*	bla_TEM−1B
Amino/β-lac inh^‡	AMC	bla_CMY−2		ampC^*	bla_CMY−2	bla_CMY−2	ampC^*	ampC^*
Cephamycin	FOX	bla_CMY−2		ampC^*	bla_CMY−2	bla_CMY−2	ampC^*	ampC^*
Cephalosporin	TIO	bla_CMY−2		ampC^*	bla_CMY−2	bla_CMY−2	ampC^*	ampC^*
	CRO	bla_CMY−2		ampC^*	bla_CMY−2	bla_CMY−2	ampC^*	ampC^*
Folate pathway	FIS	sul2	sul3	sul1, sul2	sul2	sul, sul2	sul, sul2	sul2	sul, sul2
antagonist	SXT	dfrA1	dfrA16	dfrA23	dfrA1	dfrA12	dfrA23	dfrA1	dfrA17
Macrolide	AZM					mph(A)
Phenicol	CHL	floR	cmlA1^†	floR	floR	floR	floR	floR	floR
Quinolone	DAN		gyrA^†						gyrA^†
	ENR		gyrA^†						gyrA^†
	NAL		gyrA^†						gyrA^†
Tetracycline	TET	tet(B)	tet(A)	tet(A)	tet(B)	tet(A), tet(B)	tet(A), tet(B)	tet(A)	tet(A)

Phenotypic resistance is represented by dark and light gray cells for intermediate and resistant isolates, respectively. White cells are for susceptible isolates. Resistance genes indicated in cells denote their presence for a particular isolate.

Promoter mutation in ampC−42 C -> T;

^†

Mutation in gyrA S83L;

^‡

Penicillin +β-lactamase inhibitor.

AMC, amoxicillin-clavulanic acid; AMP, ampicillin; AZM, azithromycin; CHL, chloramphenicol; CRO, ceftriaxone; DAN, danofloxacin; ENR, enrofloxacin; FIS, sulfisoxazole; FOX, cefoxitin; GEN, gentamicin; NAL, nalidixic acid; NEO, neomycin; SPT, spectinomycin; STR, streptomycin; SXT, trimethoprim-sulfamethoxazole; TET, tetracycline; TIO, ceftiofur.