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. 2021 Jun 3;11:11818. doi: 10.1038/s41598-021-91307-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Magnitude of vortex shedding correlates with eGFP expression and cell viability. eGFP-encoding mRNA was delivered to activated primary human CD3⁺ T cells via µVS with microfluidic devices containing splitter plates (SP) of various lengths. Splitter plate ratio is defined as the ratio between the length of the splitter plate and the center-to-center distance of two consecutive cylindrical posts in the flow-wise direction. Levels of (A) eGFP expression and (B) cellular viability were quantified at 24 h post-transfection. Data represent the mean ± standard deviation of n = 3 samples per condition and ≥2 independent experiments. Dot plots representative of triplicate samples with SP ratios and control indicated in each plot. *P < 0.05 by Kruskal–Wallis. Figure generated using Adobe Illustrator Creative Cloud (adobe.com/products/illustrator.html) and GraphPad Prism 9 (graphpad.com/scientific-software/prism/).