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. 2021 Jun 3;12:3319. doi: 10.1038/s41467-021-23642-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative μCT images showing the 3D bone structures of femurs from Sham, OVX, OVX-Vector, OVX-Zol, OVX-Nron, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 1 mm. b μCT measurements of BV/TV, Tb.N, and Tb.Th in femurs from Sham, OVX, OVX-Vector, OVX-Zol, OVX-Nron, and OVX-NCM2 group, (n = 6/group). c Representative H&E staining images of femurs from Sham, OVX, OVX-Vector, OVX-Zol, OVX-Nron, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 500 μm. d Representative TRAP staining images of femurs from Sham, OVX, OVX-Vector, OVX-Zol, OVX-Nron, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 500 μm (upper panels); 100 μm (lower panels). e Histological measurements of BV/TV values in c, (n = 6/group). f Quantification of osteoclastic metrics Oc.N/B.Pm and Oc.S/BS in subepiphyseal region of femurs in d, (n = 6/group). Data are presented as the means ± s.e.m. The significant differences between Sham/OVX-Vector/OVX-Zol/OVX-Nron/OVX-NCM2 treatment group with OVX group were determined by one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.