Fig. 2. Challenge test datasets.

a The overlap between Round 1 and Round 2 kinase inhibitors and kinase targets, and their distributions in the kinome tree (b), and across various kinase groups (e). c The quantitative dissociation constant (K_d) of compound-kinase activities was measured in dose-response assays (see Methods), presented in the logarithmic scale as pK_d = −log₁₀(K_d). The higher the pK_d value, the higher the inhibitory ability of a compound against a protein kinase (Supplementary Data 1 includes the compounds and kinases in Round 1 and Round 2 test datasets). The frequent values of pK_d = 5 originate from inactive pairs (maximum tested concentration of 10 µM in the multi-dose activity profiling). d The selectivity index of kinase inhibitors was calculated based on the single-dose activity assay (at 1 µM concentration) across the full compound-kinase matrices before the Challenge. The kinome tree figure was created with KinMap, reproduced courtesy of Cell Signaling Technology, Inc. Source data are provided as a Source Data file⁵⁴.