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. 2021 Jun 3;11:11676. doi: 10.1038/s41598-021-91112-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of DC-SIGN pre-exposure on anti-IgM-induced endocytosis. (a) WSU-FSCCL cells incubated with AF488-conjugated anti-IgM (5 μg/ml) at 4 or 37 °C or were left untreated for 30 min. Then cells were incubated with anti-AF488 antibody (30 min at 4 °C to quench AF488 remaining on the surface), or left untreated. Graph on the left shows cells with no pre-treatment, whereas graphs in the middle and right show results for cells that were pre-treated for 30 min at 4 °C with DC-SIGN-Fc or DC-SIGN-HA respectively, prior to anti-IgM stimulation.