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. 2021 Jun 3;11:11676. doi: 10.1038/s41598-021-91112-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of DC-SIGN on anti-IgM signaling in primary FL samples. (a, b) Anti-IgM-induced iCa²⁺ flux in primary FL samples following pre-treatment with DC-SIGN-HA. (a) Representative results of FL-B536 pre-treated with DC-SIGN-HA follow by addition (arrow) of the stimulation as stated and (b) summary of data for 4 samples. Graphs show mean (± SD) response with values for anti-IgM only cells set to 100%. Statistical significance of the indicated differences is shown (paired t-test). (c, d) Immunoblot analysis showing effect of pre-treatment with DC-SIGN-HA on total and phosphorylated LYN and SYK expression in anti-IgM-stimulated FL cells. Cells were pre-exposed to DC-SIGN-HA for 30 min at 4 °C and then stimulated for 30 s with control antibody, DC-SIGN-HA or anti-IgM at 37 °C. GAPDH expression was analyzed as loading control. (c) Representative results (FL-B536). Gels were run under the same experimental conditions and molecular weight of proteins markers shown as KDa. Full-length blots are shown in Supplementary Fig. 5. (d) Summary of data for 4 samples. Graphs show mean (± SD) response with values for no pre-treat (ie anti-IgM treated only) cells set to 100%. The statistical significance of the indicated differences is shown (paired t-test).