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. 2021 May 21;12:655652. doi: 10.3389/fphar.2021.655652

FIGURE 2.

FIGURE 2

FSGen protects IEC-6 cells from irradiation. (A) Cell Counting Kit-8 at 48 h after treatment by different concentrations of FSGen (0, 2, 4, 8, and 16 μmol/L). (B) Effect of different concentrations of FSGen (0, 2, 4, 8, and 16 μmol/L) on cell viability 24, 48, and 72 h after 6 Gy (left) or 12 Gy (right) X-ray irradiation. Cell viability was determined using the CCK-8 assay. (C–H) Vehicle (DMSO) or 4 μmol/L FSGen was added to IEC-6 cells for 24 h before 6 Gy of X-ray irradiation. After a further 72 h of culture, apoptosis was analyzed by flow cytometry (C) and by detecting apoptosis-related caspase 3 level (D). DNA damage was analyzed by SCGE (E) and by γ-H2AX staining (F). The generation of ROS and senescence was determined by DCF-DA assay (G) and by β-galactosidase staining (H). Measurement were conducted in triplicate. Bar graphs represent mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.