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. 2021 May 21;12:655652. doi: 10.3389/fphar.2021.655652

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Copyright © 2021 Zhang, Pang, Zhang, Liu, Wang, Xu, He, Li, Zhang, Zhang, Wang, Zhang, Hao, Zhang, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FSGen improves DNA damage repair by upregulating RASSF1A and ERCC1. IEC-6 cells were treated with FSGen for 48 h. (A) mRNA expression of DNA repair pathway-related genes were analyzed by Real-time PCR (two-tailed Student’s t-test, mean ± SD, n = 7). (B) RASSF1A and ERCC1 proteins were analyzed by Western blot. (C) IHC RASSF1A and ERCC1 staining of mice intestine tissues. (two-tailed Student’s t-test, mean ± SD, n = 7). (D) Correlation analysis between RASSF1A with γ-H2AX and ERCC1 with γ-H2AX in the mouse intestine. Mice were pretreated with vehicle (IR) or FSGen (FSGen+IR) and subjected to 7.5 Gy irradiation (Spearman’s rank correlation test, n = 7). (E) Bar graph showing the effect of silencing Rassf1a or/and Ercc1(siRas, siEr, siRas+siEr) on cell viability of pretreated the cells with FSGen and irradiation. (two-tailed Student’s t-test, mean ± SD, n = 7). Cell viability was determined using the CCK-8 assay. A minimum of three independent runs was performed. ^∗ p < 0.05, ^∗∗ p < 0.01, ns, not significant.