Figure 2.

HOXA13 promotes 5-FU resistance in GC cells. (A) Relative expression levels of HOXA13 in cell lines were detected by qRT-PCR. (B, C) The expression levels of HOXA13 were verified by Western blot in GC cells after transfection. (D, E) CCK-8 assays detected relative cell viability of GC cells with various concentrations of 5-FU. (F G) The rates of EdU staining in HOXA13+5-FU groups were higher than those of Vector + 5-FU groups, while knockdown of HOXA13 had the opposite effect. Magnification ×200. (H, I) After 5-FU treatment, the relative colony formation rates of HOXA13-overexpressing cells were higher than that of Vector groups, while the relative rates of colonies were reduced in HOXA13 knockdown cells. *P < 0.05, **P < 0.01, ***P < 0.001.