Skip to main content
. 2021 May 21;11:616625. doi: 10.3389/fonc.2021.616625

Figure 2.

Figure 2

AR antagonists inhibit proliferation of human and mouse GBM cell lines in vitro. (A–D) GBM cell lines were treated with indicated concentrations of enzalutamide for 2 days before cell titer blue assays were performed. (A) U87MG cell line (human) (IC50 = 41 μM). (B) U138MG cell line (human) (IC50 = 45 μM); (C) Ln229 cell line (human) (IC50 = 41 μM). (D) MGPP3 cell line (mouse) (IC50 = 56 μM); (E–H) GBM cell lines were treated with indicated concentrations of bicalutamide for 2 days before cell titer blue assays. (E) U87MG cell line (IC50 = 121 μM). (F) U138MG cell line (IC50 = 122 μM). (G) Ln229 cell line (IC50 = 72 μM). (H) MGPP3 cell line (IC50 = 42 μM). (I) Western blotting assays demonstrate that AR and c-Myc protein levels decrease in the U87MG, MGPP3 and LnCap cells (a prostate cancer cell line for positive control) after the treatment of enzalutamide (40 µM) time dependently. 8, 24, 48 h: cells were treated with the drug for 8, 24 or 48 h. All experiments were performed with three independent replicates.