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. 2021 May 21;11:609548. doi: 10.3389/fonc.2021.609548

Figure 3.

Figure 3

p62 downregulation using small interfering RNA transfection did not inhibit tumor proliferation in SPC-A-1 and A549 cells. (A) Small interfering p62 RNA (siR-p62) plasmid was successfully transfected in A549 cells. The left control indicates untreated tumor cells. The middle siR-p62 (IFF) indicates small interfering p62 RNA-transfected tumor cells in immunofluorescence field. The right siR-p62 (DF) indicates small interfering p62 RNA-transfected tumor cells in dark field (scale bar 200µm). (B) siR-p62 plasmid was successfully transfected in SPC-A-1 cells. (C) After 48h p62 mRNA expression level was downregulated in siR-p62 transfected cells than scrambled control cells (siR-NC). (D) p62 protein expression level was downregulated in siR-p62 transfected cells than scrambled control cells (siR-NC). (E, F) The difference in proliferation between siR-p62 transfected group and scrambled control group (siR-NC) of A549 (E) and SPC-A-1 (F) cells was found to be not significant between 0 h to 72 h. (G, H) Cell colony formation assay showed the difference in proliferation between siR-p62 transfected group and scrambled control group (siR-NC) of A549 cells was not significant between 0 h to 72 h (scale bar 50µm). control, untreated A549 or SPC-A-1 cells; siR-p62, small interfering p62 RNA transfected cells; siR-NC, siR-p62 scrambled control; IFF, immunofluorescence field; DF, dark field; **P < 0.01.