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. 2021 May 21;11:609548. doi: 10.3389/fonc.2021.609548

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Copyright © 2021 Li, He, Ye, Ye, He, Chen and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo p62 downregulation had no effect on growth of subcutaneous tumor. (A) The difference of tumor volumes between silenced p62 group (n = 3), scrambled control group (n = 2) and control group (n = 2) was not statistically significant. (B) All mice model was dissected on the 28th day, and lung or bone metastasis lesion was not found. (C) Bone metastasis lesion of limb or spine was not found under microscope (scale bar 200µm). Ctrl, untreated A549 cells; shR-p62, stably transfected A549 cells; shR-NC, shR-p62 scrambled control.