Figure 2.

Effect of the intravenous (IV) administration of two different doses of the lactate receptor agonist 3-chloro-5-hydroxybenzoic acid (CHBA) after 30 min MCAO. (A) Direct (left) and indirect (right) lesion volumes and (B) percentage of swelling from brains of mice that received an IV injection of vehicle (PBS, n = 7), a low dose (25 nmol/g, n = 5) or a high dose (0.1 μmol/g, n = 5) of CHBA at reperfusion. Measurements were done on cresyl violet-stained serial coronal sections of frozen brains from mice sacrificed 48 h after MCAO induction. To evaluate the functional outcome following IV treatment with PBS or CHBA, mice were scored for (C) neurological deficit (neuroscores: 0, no deficit; 1, failure to extend right forepaw; 1.5, intermittent circling; 2, circling; and 3, loss of circling or righting reflex), (D) performance on Rotarod, (E) overall performance on wire-hanging test, (F) number of falls from the wire, (G) number of times mice escaped from the wire, and (H) maximal latency time to fall from the wire. All the behavioral measurements were recorded at 24 h and 48 h after MCAO. Filled dots represent individual animals.