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. 2021 May 21;12:689239. doi: 10.3389/fphys.2021.689239

Figure 4.

Figure 4

Effect of the intracerebroventricular (ICV) administration of the lactate receptor agonist CHBA after 30 min MCAO. (A) Direct (left) and indirect (right) lesion volumes and (B) swelling volumes from brains of mice that received a 2 μl ICV injection of vehicle (PBS, n = 6) or 1 mmol/L CHBA (n = 8) at reperfusion. Measurements were done on cresyl violet-stained serial coronal sections of frozen brains from mice sacrificed 48 h after MCAO induction. To evaluate the functional outcome following ICV treatment with PBS or CHBA, mice were scored for (C) neurological deficit (neuroscores: 0, no deficit; 1, failure to extend right forepaw; 1.5, intermittent circling; 2, circling; and 3, loss of circling or righting reflex), (D) overall performance on wire-hanging test, (E) number of falls from the wire, (F) number of times mice escaped from the wire, and (G) maximal latency time to fall from the wire. All the behavioral measurements were recorded at 24 h and 48 h after MCAO. Filled dots represent individual animals.