Figure 1.

EH_ITSN induces significant differences in pulmonary artery remodeling between female (F) and male (M) EH_ITSN-KO^ITSN+/– mice. Representative hematoxylin/eosin staining of mouse lung sections illustrates the vascular arteriopathy and pulmonary artery (PA), different diameter, remodeling in female (A–C) and male (F–H) EH_ITSN-KO^ITSN+/– mice. Lung sections of wild-type (wt) female (D and E) and male (I and J) mice illustrate representative pulmonary arteries of different diameter used for comparison. Quantification of vascular lesions (K) and morphometric analyses of small (20 to 50 μm) and medium (50 to 100 μm) pulmonary artery muscularization in wt female and wt male mice, as well as in female and male EH_ITSN-KO^ITSN+/– mice (L). The morphometric analyses of small and medium pulmonary artery muscularization for wt male and wt female mice show aggregated (20 to 100 μm diameter) data. The number of lesions is reported per three mouse lung cross-sections. Low magnifications of the lung images, female and male wt mice (M and N) and EH_ITSN-KO^ITSN+/– mice (O and P), are also shown. Arrows (M and N) indicate vascular profiles. n = 3 mice for each wt male and wt female data (D, E, I, J, M, and N); n = 9 mice for each female and male, with 3 slides per mouse, per experimental condition (A–C, F–H, O, and P). ∗P < 0.05, ∗∗P < 0.01. Scale bars: 20 μm (A–J); 40 μm (M–P). Original magnification, ×20 (M–P).