Table 1.
The Primer Forward and Reverse Sequences for lncRNA-Xist and Human Elk1 (Sigma-Aldrich), Mouse and Human GAPDH and Mouse Elk1 (Origene), Rat Elk1, Mouse and Rat Cyclin A1, and Rat GAPDH (Gene Copoeia, Inc.), and Human lncRNA Xist (BioRad), Used for qPCR Analysis
| Oligonucleotide name (catalog no.) |
Forward sequence | Reverse sequence |
|---|---|---|
| lncRNA Xist (rat) | 5′-TGCCTGGATTTAGAGGAG-3′Oligonucleotide no. 3023101015–000030 | 5′-CTCCACCTAGGGATCGTAA-3′Oligonucleotide no. 3023101015–000040 |
| lncRNA Xist (mouse) | 5′-GCCCAAAGGGACAAACAATC-3′Oligonucleotide no. 3022576202–000010 | 5′-GTAGCGAGGACTTGAAGAGAAG-3′Oligonucleotide no. 3022576202–000020 |
| lncRNA Xist (human) (catalog no. 12004176) |
5′-GGATGTCAAAAGATCGGCCC-3′ | 5′-GTCCTCAGGTCTCACATGCT-3′ |
| Elk1 (rat) (catalog no. RQP067189) |
5′-GGCTTCAGTCCAGAAGCTGT-3′ | 5′-GGGGTTGGGTGATCTCAGTG-3′ |
| Elk1 (mouse) (catalog no. MP204140) |
5′-GGAACAAGCTCTGGTCTTCAGG-3′ | 5′-GGACTCAGAGTGCTCCAGAAATG-3′ |
| Elk1 (human) | 5′-GCTGCCTCCTAGCATTCACTTC-3′Oligonucleotide no. 3022585344–000130 | 5′-CCACGCTGATAGAAGGGATGTG-3′Oligonucleotide no. 3022585344000140 |
| Cyclin A1 (rat) (catalog no. RQP046089) |
5′-ACCGTGCTAGGAGTGTTGAC-3′ | 5′-CCAGCTGCAGGGAAGACATT-3′ |
| Cyclin A1 (mouse) (catalog no. 026720MQP) |
5′-TGAGCCTTCTGGAAGCTGAC-3′ | 5′-ACTCAGGCAAGGCACAATCT-3′ |
| GAPDH (rat) (catalog no. RQP049537) |
5′-CAGCCGCATCTTCTTGTGC-3′ | 5′-GGTAACCAGGCGTCCGATA-3′ |
| GAPDH (mouse) (catalog no. MP205604) |
5′-CATCACTGCCACCCAGAAGACTG-3′ | 5′-ATGCCAGTGAGCTTCCCGTTCAG-3′ |
| GAPDH (human) (catalog no. HP205798) |
5′-GTCTCCTCTGACTTCAACAGCG-3′ | 5′-ACCAACCCTGTTGCTGTAGCCAA-3′ |
Elk1, ETS domain containing protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; lncRNA, long noncoding RNA; qPCR, real-time quantitative PCR.