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. 2021 Jun;191(6):1108–1117. doi: 10.1016/j.ajpath.2021.02.016

Figure 2.

Figure 2

Enhanced distribution and activation of mast cells at the nasal side of the cornea. A: Representative immunohistochemistry micrograph of BALB/c murine cornea whole mounted and stained with fluorescent-conjugated avidin (Texas red) to visualize mast cells. B: Cumulative bar chart showing the number of mast cells (avidin+) on the nasal and temporal side of the cornea. The cornea was cut into nasal and temporal halves at harvesting. ImageJ version 1.52v software was used to quantify the number of mast cells. C: Levels of tryptase (left side) and β-hexosaminidase (right side) of lysates of nasal and temporal halves of naïve corneas. D: Ocular surface tear wash was collected at 0, 1, 3, and 6 hours following nasal or temporal suture placement (5 μL/wash) to measure mast cell activation markers tryptase (left side) and β-hexosaminidase (right side). Tear wash was collected from naïve mice as control. Representative data from three independent experiments are shown, and each experiment consisted of four animals. Data are represented as means ± SEM (B–D). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (t-test). Scale bar = 100 μm (A).