Figure 3.

Comparable neovascularization at nasal and temporal cornea in mast cell–deficient mice. A: Representative immunohistochemistry micrograph of cKit^w-sh mice cornea whole mounted and stained with fluorescent-conjugated avidin (Texas red) to visualize deficiency of mast cells. B: Representative slit-lamp biomicroscope images of nasal or temporal sutured corneas of wild-type (WT; C57BL/6) and cKit^w-sh mice on day 0 (naïve cornea), 2, and 4 after suture. C: Binary images (left side) and cumulative bar chart (right side) depicting the vascular density of corneal neovascularization. Slit-lamp images were converted into binary images, and vascular density as percentage area of the vessels in the total cornea was calculated using the vessel analysis plugin in ImageJ version 1.52v software. Representative data from three independent experiments are shown, and each experiment consisted of three animals. Data are represented as means ± SEM (C). ∗P < 0.05 (t-test). Scale bar = 100 μm (A). Sut., suture.