Supplemental Figure S1.

Fluorescent intensity of Dkk1 protein expression in glaucomatous trabecular meshwork (GTM) and non-glaucomatous trabecular meshwork (NTM). The TM region, as shown in Figure 2, was manually selected, and the fluorescent intensity of Dkk1 and β-tubulin was measured using the ImageJ version 1.8.0_172 software. Dkk1 fluorescent intensity was normalized to β-tubulin fluorescent intensity (relatively fluorescent intensity), and the data were plotted. Data were analyzed using t-test. Data are given as means ± SD of Dkk1 relative fluorescent intensity. N = 3 for GTM; N = 6 for NTM. ∗P < 0.05.