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. 2021 Jun;191(6):1020–1035. doi: 10.1016/j.ajpath.2021.02.018

Figure 3.

Figure 3

Dkk1 plays a positive role in glucocorticoid receptor (GR) signaling in the human trabecular meshwork (TM). GTM3 cells (A and C) were cotransfected with the glucocorticoid receptor signaling reporter vector together with pcDNA3.1-empty/Dkk1 vector or nontargeting (NT) siRNA/Dkk1 siRNA to overexpress (A) or knock down (C) Dkk1, respectively. Similarly, GRE-NTM5 (B and D), which were stably transduced with glucocorticoid receptor signaling reporter cassettes, were treated/transfected with phosphate-buffered saline (PBS)/recombinant Dkk1 (rDkk1; 200 ng/mL) or NT siRNA/Dkk1 siRNA. All cells were treated with 0.1% ethanol (EtOH) or 100 nmol/L dexamethasone (Dex) before luciferase assays. Data were analyzed using one-way analysis of variance and Sidak post-hoc tests. Data are given as means ± SD of GR signaling activity (A–D). N = 6 (A and C); N = 8 (B and D). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.