Figure 2.

Myeloid–hypoxia-inducible factor-1α (HIF1α) deficiency reshapes cellular components of stenotic arteries. The Lyz2^cre/cre and Hif1α^fl/fl:Lyz2^cre/cre mice control and ligated (28 days) carotid artery cross-sections were stained for vascular smooth muscle cells (A) and macrophages (B) by using anti–smooth muscle actin and anti-F4/80 antibodies, respectively. The vascular smooth muscle cell (C) and macrophage (D) areas were quantified using ImageJ software. Data were analyzed by analysis of variance, followed by Bonferroni post-testing. Values are reported as means ± SD (C and D). n = 8 (A–D). ∗∗∗P < 0.001. Scale bar = 100 μm (A and B).