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. 2021 Jun;191(6):1118–1134. doi: 10.1016/j.ajpath.2021.03.008

Figure 8.

Figure 8

STAT3–hypoxia-inducible factor-1α (HIF1α) axis modulates inflammatory gene expression in macrophages. A: RAW264.7 cells were transfected with an HIF1α promoter-driven luciferase reporter construct in the presence of control or Stat3-specific siRNA. These cells were stimulated with lipopolysaccharide (LPS) or interferon (IFN)-γ for 6 hours, and cell lysates were analyzed for luciferase activity. B: Wild-type mice bone marrow–derived macrophages were stimulated with LPS or IFN-γ for 4 hours, and chromatin immunoprecipitation analysis was performed on Hif1α promoter (−1041 to −1051) utilizing anti-STAT3 antibody. C–F: RAW264.7 cells were cotransfected with a combination of Stat3-specific siRNA or oxygen-stable form ΔHIF1α plasmid and stimulated with 100 ng/mL LPS for 5 hours. C–F: Total RNA from these experiments was evaluated for expression of Il1α (C), Il1β (D), Icam1 (E), and Serpine1 (F) by RT-qPCR. Data were analyzed by analysis of variance, followed by Bonferroni post-testing. Values are reported as means ± SD (A–F). n = 3 (A and B); n = 4 (C–F). ∗∗P < 0.01, ∗∗∗P < 0.001. PBS, phosphate-buffered saline.