FIGURE 6.

Protegrin-1 increases cell proliferation might via upregulating phosphorylation of EGFR (pEGFR) in cultured granulosa cells. (A) Western Blot analysis for relative expression of pEGFR, EGFR and GAPDH protein. (B) pEGFR inhibitor AG1478 on Protegrin-1-induced granulosa cell proliferation. SGCs were pretreat with 20 μM AG1478 for 30 min or unpretreat, then incubated with 1 μg/ml Protegrin-1 for 24 h and cell proliferation was evaluated by cell counting. The data shown are representative of three independent experiments. Data represent the mean ± SEM of three independent experiments *P < 0.05 compared to control.