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. 2021 May 5;12(13):3909–3919. doi: 10.7150/jca.55666

Figure 6.

Figure 6

LINC01272 bound to miR-876 and miR-876 targeted ITGB2 in CRC. a, Prediction analysis with the DIANA lab database showed that LINC01272 contained a miR-876-binding sequence. Based on the above, a LINC01272-Luc WT plasmid and LINC01272-Luc MT plasmid were constructed. b and c, Bioinformatics and qRT-PCR analyses were performed to detect LINC01272 and miR-876 and showed that these two factors were negatively associated (P<0.05). d, Based on prediction analysis of the miRbase, miRecords and TargetScan databases, the ITGB2 3'UTR contained a miR-876-binding sequence. e, A LINC01272-Luc WT plasmid, LINC01272-Luc MT plasmid and their corresponding controls were transfected into HCT116 cells. Transwell assays were carried out and showed that miR-876 inhibited the luciferase activity of the LINC01272-Luc WT plasmid but not the luciferase activity of the LINC01272-Luc MT plasmid in HCT116 cells. Similarly, Transwell assays were performed and showed that miR-876 inhibited the luciferase activity of the ITGB2-Luc WT plasmid but not the luciferase activity of the ITGB2-Luc MT plasmid in HCT116 cells. Three independent experiments were performed. *p <0.05, **p < 0.01 compared to the control group by Student's t-test.