Effect of VES on CD47 and calreticulin expression of human cervical cancer cells. (A) HeLa, CaSki and SiHa cancer cells were treated with 5, 7.5 and 10 µg/mL VES for 12 h, and the expression of CD47 was examined by quantitative real-time PCR. (B) HeLa cancer cells were treated with 5, 7.5 and 10 µg/mL VES for 12 h, and the expression of calreticulin was examined by quantitative real-time PCR. (C) Cells were treated with 5, 7.5 and 10 µg/mL VES for 24 h and subjected to flow cytometry. Black: blank control, red: control group, blue: 5 µg/mL VES group, orange: 7.5 µg/mL VES group, green: 10 µg/mL VES group. The data are representative of at least 3 independent experiments. *P ≤ 0.05 compared with control.