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. 2021 May 13;12(13):4099–4108. doi: 10.7150/jca.58097

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FIRRE positively regulates PFKFB4 expression in HCC cells. (A) shNT or shFIRRE-1/2 was transfected into HCCLM3 cells, and qRT-PCR and immunoblotting were performed to analyze PFKFB4 and PKM2 expression. (B) OE-FIRRE or empty vector (EV) was transfected into Huh7 cells, and qRT-PCR and immunoblotting were carried out to detect PFKFB4 and PKM2 expression. (C) The expression of PFKFB4 mRNA in eighty pairs of HCC and adjacent nontumor tissues (NT) was assessed by qRT-PCR analysis. (D) TCGA data from the GEPIA website indicated the upregulated expression of PFKFB4 mRNA in HCC tissues. (E) A positive correlation between FIRRE and PFKFB4 mRNA level was observed in HCC tissues. (F) TCGA data from the GEPIA website demonstrated that the high PFKFB4 expression indicated poor overall survival and disease-free survival of HCC patients. *P<0.05.