Fig. 6.

Tumoricidal function of CAR-modified T cells from patients with ESCC. (A) Peripheral T cells isolated from patients with ESCC were transduced and CAR expression was detected 12 days later. (B) Expression of CD276 on healthy epithelial cells or tumor cells from patients with ESCC. (C and D) Primary tumor cells (EpCAM⁺CD3⁻) were purified and co-cultured with indicated CAR-T cells (EpCAM⁻CD3⁺) at E/T = 1 without exogenous cytokines overnight. Ratios of the residual tumor and T cells were determined by FACS (C). T cells were further analyzed for CD107a and IFN-γ expressions (D). ANOVA tests were performed. Data represent independent experiments with samples from three patients. ***, P < 0.005.