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. 2021 May 13;12(14):4148–4171. doi: 10.7150/jca.58076

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Single‑cell RNA‑seq analysis reveals high cell heterogeneity. A. Cells from laryngeal cancer were all annotated by CellMarker and singleR. B. Trajectory analysis revealed laryngocarcinoma cells with distinct differentiation patterns. C-D. Trajectory analysis revealed the differential expression of pRS-related genes (CFLAR, DAPK2, TSC2, CAPN10, MBTPS2, PEX14, FADD, ST13 and HAS2) at different pseudo-time. E. 8 pRS-related genes (CFLAR, DAPK2, TSC2, CAPN10, MBTPS2, PEX14, FADD and ST13) clustering analysis divided laryngocarcinoma cells into two clusters (the low risk cell cluster and the high risk cell cluster) with a well discriminatory power. F. A bar chart of cell classification percentage in the low risk cell cluster and the high risk cell cluster.