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. 2021 May 17;12(14):4218–4228. doi: 10.7150/jca.47506

Figure 5.

Figure 5

The exogenous expression of NMHC IIA enhanced the migration and invasiveness in COS7 cells. A. Quantitative real time PCR analysis of MYH9 gene in COS7 cells expressing control vector PLNCX2 or PLNCX2/NMHC IIA. The relative fold increase of transcripts was normalized to the amount of RNA harvested from cells expressing control vector PLNCX2. GAPDH served as the internal control. The data were presented as the mean ± SD (n = 3). B. Western blot analysis of NMHC IIA protein in COS7 cells expressing control vector PLNCX2 or PLNCX2/NMHC IIA. β-actin was used as a loading control. C. The migratory and invasive abilities induced by NMHC IIA were analyzed in COS7 cells expressing control vector PLNCX2 or PLNCX2/NMHC IIA. The migratory or invasive cells were stained by crystal violet and then photographed by fluorescence inversion microscope system (200×). E. Migratory cells or invasive cells were plotted as the average number of cells per field of view from ten random fields.