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. 2021 May 13;12(14):4196–4208. doi: 10.7150/jca.58522

Figure 2.

Figure 2

Loss of miR-1246 in drug-resistant leukemia cells could improve their drug sensitivity and induce cell apoptosis. A, The expression of miR-1246 was inhibited in chemo-resistant leukemia cells (* P < 0.05, comparing to their negative control, respectively). B, The cell survival and proliferation rate was measured using MTT assay after the miR-1246 was repressed in K562/ADM and HL-60/RS cells (* P < 0.05, ** P < 0.01, comparing to the NC inhibitor transfected cells treated with the same concentration of ADM, respectively). C, Morphological alterations of K562/ADM and HL-60/RS cells transfected with NC inhibitor or miR-1246 inhibitor and treated with or without ADM. (a) Morphological changes detected by phase-contrast microscopy (×200). (b) Ultrastructural changes of cells dectected by transmission electron microscopy (×10,000). D, The cell apoptotic rate was examined using flow cytometry staining with Annexin V-FITC and PI (* P < 0.05, comparing to their negative control. # P < 0.05, comparing to their negative control treated with the same concentration of ADM). E, After the cells were fixed and stained, flow cytometry was used to check the cell cycle distribution. F, Relative positive rate and mean fluorescence intensity were analysed to investigate the ADM efflux ability of cells using flow cytometry. (* P < 0.05, ** P < 0.01, comparing to their negative control, respectively).