. 2021 May 3;104(6):2152–2158. doi: 10.4269/ajtmh.21-0047

Table 1.

Primers and PCR conditions used for the genotyping of ABCB1, ABCG2, and ABCC1 polymorphisms

Gene/SNPs	Primer sequence	PCR condition denaturation/annealing/extension	Enzyme
MDR1, c.1236C>T (rs1128503)	F: 5′-TGTGTCTGTGAATTGCCTTGA-3′	94°C 60 s/56°C 60 s/72°C 60 s	HaeIII, 37°C/16 h
MDR1, c.1236C>T (rs1128503)	R: 5′-ATCTCACCATCCCCTCTGTG-3′	94°C 60 s/56°C 60 s/72°C 60 s	HaeIII, 37°C/16 h
MDR1, c.2677G>T (rs2032582)	F: 5′-TGCAGGCTATAGGTTCCAGG-3′	94°C 30 s/58°C 30 s/72°C 45 s	BanI, 37°C/16 h
MDR1, c.2677G>T (rs2032582)	R: 5′-TTTAGTTTGACTCACCTTCCCG-3′	94°C 30 s/58°C 30 s/72°C 45 s	BanI, 37°C/16 h
MDR1, c.3435C>T (rs1045642)	F: 5′-TTGATGGCAAAGAAATAAAGC-3′	94°C 90 s/56°C 60 s/72°C 90 s	MboI, 37°C/16 h
MDR1, c.3435C>T (rs1045642)	R: 5′-CTTACATTAGGCAGTGACTCG-3′	94°C 90 s/56°C 60 s/72°C 90 s	MboI, 37°C/16 h
BCRP, c.34G>A (rs2231137)	F: 5′-CAGTAATGTCGAAGTTTTTATCGCA-3′	94°C 30 s/58°C 30 s/72°C 60 s	BseMI, 55°C/16 h
BCRP, c.34G>A (rs2231137)	R: 5′-AAATGTTCATAGCCAGTTTCTTGGA-3′	94°C 30 s/58°C 30 s/72°C 60 s	BseMI, 55°C/16 h
BCRP, c.421C>A (rs2231142)	F: 5′-GTTGTGATGGGCACTCTGATGGT-3′	94°C 30 s/58°C 30 s/72°C 60 s	TaaI, 65°C/16 h
BCRP, c.421C>A (rs2231142)	R: 5′-CAAGCCACTTTTCTCATTGTT-3′	94°C 30 s/58°C 30 s/72°C 60 s	TaaI, 65°C/16 h
MRP1, c.218C>T (rs41494447)	F: 5′-TCAGATGACACCTCTCAACAGAA-3′	94°C 30 s/56.5°C 30 s/72°C 30 s	Hinf I, 37°C/16 h
MRP1, c.218C>T (rs41494447)	R: 5′-CCAGTTTTCACCTCCCACATTAT-3′	94°C 30 s/56.5°C 30 s/72°C 30 s	Hinf I, 37°C/16 h
MRP1, c.2168G>A (rs4148356)	F: 5′-GCCTGGATTCAGAATGATTCTCTTC-3′	94°C 30 s/52°C 30 s/72°C 30 s	Taq I, 65°C/16 h
MRP1, c.2168G>A (rs4148356)	R: 5′-TACTGACCTTCTCGCCAATCTCTGT-3′	94°C 30 s/52°C 30 s/72°C 30 s	Taq I, 65°C/16 h
MRP1, c.3173G>A (rs41410450)	F: 5′-TCTGCATTGTGGAGTTTT-3′	94°C 60 s/53°C 60 s/72°C 60 s	Pst I, 37°C/16 h
MRP1, c.3173G>A (rs41410450)	R: 5′-GACGAAGAAGTAGATGAGGC-3′	94°C 60 s/53°C 60 s/72°C 60 s	Pst I, 37°C/16 h