Figure 2. Bone Marrow Niches.

(A) Prior to scRNA-seq studies, BM vascular and perivascular Lepr⁺ cells were identified as the key populations constituting the HSC niche. The degree of the heterogeneity within those cellular subsets was unknown.

(B) scRNA-seq of BM vascular endothelial and perivascular Lepr⁺ cells resolved cellular composition of these populations and defined expression patterns of pro-hematopoietic growth factors, chemokines, and membrane-bound ligands.

(C) Changes in gene expressionin BM vascular endothelial and perivascula Lepr⁺ cells following acute chemotherapy treatment.