Fig. 6.

YTHDF1 promoted the migration and invasion of HCC cells via inducing EMT. A Western blot was performed to detect the influence of si-YTHDF1 on protein levels of EMT markers in Huh7 and MHCC-97H cells. B The MHCC-97H cells with YTHDF1 knockdown were treated with 10 ng/ml TGF-β for 48 h, then measured the protein levels of EMT markers by western blot. C Transwell assay and D wound healing assay were performed to assess the migration and invasion ability of MHCC-97H cells with YTHDF1 silencing that were pretreated with 10 ng/ml TGF-β for 48 h. E Western blot was performed to detect the protein levels of Claudin 1 and Vimentin in MHCC-97H cells treated with or without 4ug/ml SC79 for 1 h upon YTHDF1 silencing. Representative images and quantitative histograms were shown. *P < 0.05, **P < 0.01, ***P < 0.001