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. 2021 Jun 4;22(Suppl 3):405. doi: 10.1186/s12864-021-07717-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Validating the expressions of some MacERV3 LTRs with PCR experiments. (A) The relative expressions of 13 MacERV3 LTRs detected with the qRT-PCR experiments. (B) The expressions of the two MacERV3 LTRs in Part (A) detected with RNA-Seq profiles. One of the two replicated RNA-Seq profiles for each lines were shown. (C) The expressions of the LTRs in Part (A) examined with semi-quantitative RT-PCR in the samples used. GAPDH was used as an internal control. Three replicates (r1 to r3) were included for each of the 7 cell lines. (D) The sequence and scores of nucleotides of the PCR product. (E) The genomic loci of MacERV3_LTR2_34. The positions of the PCR product and primers were shown in different lanes below MacERV3_LTR2_34