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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Exp Eye Res. 2020 Jun 6;197:108102. doi: 10.1016/j.exer.2020.108102

Figure 3. The number of degenerating ON axons and the number of ED1+ cells increased after rPBI.

Figure 3.

Total number of intact and degenerating axons were counted in the ON in blast blocked and rPBI treated eyes after intravitreal injections of vehicle and Nec-1s. A) Representative PPD and toluidine blue stained ON semi-thin cross sections showing degenerating axons (arrowheads). B) The number of intact axons was not significantly changed at 28 dpi (P = 0.1729). C) The number of degenerating axons in the ON increased after rPBI compared to blast blocked (P = 0.0014; P = 0.0008 post hoc). There were no differences between Nec-1s or vehicle treated eyes (P = 0.9862, post hoc). D) The axon density was not statistically different 28 dpi (P = 0.4539). E) The total ON area was not changed after injury or Nec-1s treatment (P = 0.3120). Error bars represent mean ± SEM. n = 10 per group. F) Immunostaining of ED1+ cells in the ON. G) The number of ED1+ cells in the ON increased after rPBI with no intravitreal injections compared to blast blocked (P = 0.0057; P = 0.0166, post-hoc) and was also more frequent compared to blast blocked after rPBI with vehicle treatment (P = 0.0064) and Nec-1s treatment (P = 0.0344). Fig. 4A scale bar represents 20 μm and Fig. 4F scale bar represents 100 μm. Error bars represent mean ± SEM. ***P < 0.001, *P < 0.05.