Fig. 4.

Atg8 acts downstream of ESCRT proteins in EVT. a, b The degradation of GFP-Pho8 is defective in ESCRT mutants. Cells were treated as Fig. 1a. Data are presented as in Fig. 1a and b, respectively. c, d The MVB pathway does not depend on Atg8. c Sorting of GFP-Cps1 monitored by fluorescent microscopy. Mid-log phase yeast cells were collected. d Processing of GFP-Cps1 into free GFP monitored by immuno-blot. e, f Epistatic analysis of atg8Δ and ESCRT mutants. GFP-Pho8 expressing cells with the indicated genotypes were treated as Fig. 1a. Data are presented as in Fig. 1a and b, respectively. See Fig. S6B for quantification of immune-blots