Table 4.

Summary of optogenetic effects on behavior metrics

	PV+ neurons, ChR2 (n = 12) Fold change	pc	Npas1+ neurons, ChR2 (n = 16) Fold change	p	Kcng4+ neurons, ChR2 (n = 8) Fold change	p	Foxp2+ neurons, ChR2 (n = 18) Fold change	p	STN neurons, ACR2 (n = 10) Fold change	p	dSPNs, ChR2 (n = 9) Fold change	p	iSPNs, ChR2 (n = 11) Fold change	p
Speed (cm/s)	1.53 ± 0.31	0.00098	0.81 ± 0.09	0.000031	1.52 ± 0.33	0.0078	0.36 ± 0.12	0.0000076	1.68 ± 0.15	0.0020	1.9 ± 0.25	0.0039	0.52 ± 0.09	0.00098
Body length-width ratio	1.1 ± 0.05	0.00049	1.0 ± 0.05	0.00015	1.16 ± 0.08	0.023	0.88 ± 0.06	0.000023	1.19 ± 0.03	0.0020	1.14 ± 0.11	0.0039	0.97 ± 0.04	0.083
Total frequencya	0.71 ± 0.12	0.00049	1.08 ± 0.08	0.000061	0.79 ± 0.06	0.0078	1.36 ± 0.17	0.000015	0.68 ± 0.06	0.0020	0.76 ± 0.16	0.0039	1.12 ± 0.09	0.31
Locomotion frequency	0.78 ± 0.12	0.00049	1.1 ± 0.04	0.000092	0.82 ± 0.1	0.023	1.32 ± 0.18	0.000076	0.71 ± 0.05	0.0020	0.91 ± 0.18	0.20	0.9 ± 0.04	0.0098
Fine movement frequency	0.69 ± 0.12	0.00049	1.06 ± 0.1	0.000061	0.77 ± 0.13	0.0078	1.35 ± 0.21	0.000015	0.63 ± 0.08	0.0020	0.82 ± 0.14	0.0039	1.04 ± 0.08	0.81
Rearing frequency	0.91 ± 0.2	0.15	0.87 ± 0.18	0.026	0.98 ± 0.13	0.56	0.68 ± 0.31	0.00092	1.56 ± 0.33	0.0039	0.97 ± 0.32	0.97	0.29 ± 0.11	0.00098
Motionless frequency	0.5 ± 0.18	0.0015	1.03 ± 0.46	0.0011	0.67 ± 0.14	0.016	1.77 ± 0.34	0.0019	0.51 ± 0.13	0.0059	0.33 ± 0.14	0.0039	1.88 ± 0.34	0.00098
Locomotion % time	1.24 ± 0.09	0.0024	0.99 ± 0.07	0.0013	1.11 ± 0.04	0.0078	0.86 ± 0.06	0.0023	1.18 ± 0.13	0.0059	1.37 ± 0.12	0.0039	0.66 ± 0.1	0.0029
Fine movement % time	0.66 ± 0.14	0.00049	1.09 ± 0.14	0.000031	0.74 ± 0.16	0.0078	1.51 ± 0.32	0.000038	0.53 ± 0.12	0.0020	0.79 ± 0.13	0.0039	1.03 ± 0.08	0.64
Rearing % time	1.02 ± 0.25	0.91	0.82 ± 0.16	0.013	1.21 ± 0.14	0.023	0.54 ± 0.25	0.0010	1.42 ± 0.25	0.020	1.11 ± 0.57	0.65	0.39 ± 0.18	0.00098
Motionless % time	0.41 ± 0.15	0.0024	1.08 ± 0.49	0.0063	0.63 ± 0.2	0.0078	1.43 ± 0.53	0.060	0.5 ± 0.19	0.020	0.18 ± 0.1	0.0039	2.84 ± 0.34	0.00098
Locomotion durationb	1.83 ± 0.36	0.00049	0.91 ± 0.05	0.000031	1.38 ± 0.17	0.0078	0.57 ± 0.08	0.000015	1.58 ± 0.21	0.0020	1.7 ± 0.41	0.0039	0.95 ± 0.23	0.21
Fine movement duration	0.96 ± 0.03	0.016	1.01 ± 0.06	0.011	0.94 ± 0.04	0.039	1.16 ± 0.1	0.0010	0.85 ± 0.08	0.0098	0.93 ± 0.07	0.027	0.94 ± 0.05	0.019
Rearing duration	1.17 ± 0.13	0.11	0.99 ± 0.11	0.074	1.34 ± 0.49	0.31	0.7 ± 0.12	0.0066	0.92 ± 0.12	0.16	0.84 ± 0.29	0.82	1.28 ± 0.56	0.43
Motionless duration	0.78 ± 0.1	0.0093	0.95 ± 0.09	0.86	0.95 ± 0.13	0.38	0.88 ± 0.11	0.067	1.0 ± 0.28	0.63	0.61 ± 0.08	0.0039	1.67 ± 0.32	0.0020
Locomotion-fine movement frequency	0.77 ± 0.14	0.00049	1.08 ± 0.08	0.000061	0.79 ± 0.17	0.039	1.32 ± 0.26	0.00033	0.62 ± 0.04	0.0020	1.01 ± 0.24	0.50	0.72 ± 0.13	0.0020
Locomotion-rearing frequency	1.02 ± 0.13	0.93	1.0 ± 0.18	0.75	0.87 ± 0.11	0.054	0.76 ± 0.11	0.083	1.43 ± 0.07	0.0020	1.14 ± 0.13	0.31	0.57 ± 0.29	0.031
Locomotion-motionless frequency	0.7 ± 0.21	0.034	1.06 ± 0.31	0.021	0.88 ± 0.25	0.64	1.37 ± 0.35	0.0019	0.73 ± 0.21	0.29	0.77 ± 0.06	0.0039	1.38 ± 0.35	0.0088
Fine movement-locomotion frequency	0.77 ± 0.11	0.00049	1.06 ± 0.11	0.00015	0.79 ± 0.16	0.039	1.35 ± 0.24	0.00011	0.64 ± 0.08	0.0020	1.02 ± 0.3	0.48	0.8 ± 0.12	0.0020
Fine movement-rearing frequency	0.95 ± 0.08	0.12	1.0 ± 0.22	0.39	0.99 ± 0.08	>0.99	0.96 ± 0.16	0.54	0.97 ± 0.2	0.47	0.86 ± 0.46	0.44	0.0 ± 0.0	>0.99
Fine movement-motionless frequency	0.71 ± 0.15	0.034	1.06 ± 0.2	0.034	0.72 ± 0.12	0.078	1.31 ± 0.36	0.048	0.6 ± 0.11	0.063	0.45 ± 0.11	0.0039	1.56 ± 0.36	0.00098
Rearing-locomotion frequency	1.09 ± 0.16	0.83	1.03 ± 0.18	0.9	0.95 ± 0.16	0.74	0.81 ± 0.19	0.086	1.17 ± 0.06	0.027	1.07 ± 0.18	0.81	0.67 ± 0.17	0.0078
Rearing-fine movement frequency	1.0 ± 0.0	0.25	1.0 ± 0.15	0.57	0.95 ± 0.06	0.13	0.9 ± 0.13	0.49	0.78 ± 0.28	0.13	1.0 ± 0.14	0.63	1.0 ± 0.33	>0.99
Motionless-locomotion frequency	0.71 ± 0.13	0.077	1.01 ± 0.33	0.0082	0.92 ± 0.17	0.74	1.31 ± 0.33	0.054	0.78 ± 0.13	0.027	0.71 ± 0.07	0.0078	1.42 ± 0.27	0.0059
Motionless-fine movement frequency	0.72 ± 0.12	0.027	0.96 ± 0.39	0.074	0.71 ± 0.09	0.023	1.36 ± 0.25	0.027	0.72 ± 0.31	0.29	0.43 ± 0.11	0.0039	1.67 ± 0.35	0.00098

^aUnit for all frequencies is count/10 s.

^bUnit for all durations is seconds.

^cWilcoxon test; two-tailed exact p values are shown.

The bolded p values are significant when using a Bonferroni correction at a significance level of 0.05.