The antidepressant effects of intra-LHb injection of (2R,6R)-HNK in CRS rats. A, Schematic illustration of experimental protocol. B, The verified infusion sites. C, Effect of intra-LHb injection of (2R,6R)-HNK on the LMA. n.s., Not significant. n = 6–8. D, Effect of intra-LHb injection of (2R,6R)-HNK on the immobile time in the FST. n.s., Not significant. n = 6–8. E, Effect of intra-LHb injection of (2R,6R)-HNK on the latency to immobility onset of FST. n.s., Not significant. n = 6 and 7. F, Effect of intra-LHb injection of (2R,6R)-HNK on the SPT. n.s., Not significant. n = 6–8. Three rats were removed from the analysis for incorrect cannulae placement (C–F). LMA, Locomotion activity.