Figure 3.

Antiserum directed to the C terminus of PKC98E detects kinase protein in the olfactory neuron dendrites. A, Cartoon depicting the Drosophila antenna with location of different sensilla. B, Cartoon showing the olfactory neuron anatomy of the cVA responsive at1 trichoid neuron. C, Depiction of the branched basiconic olfactory neuron dendrites. D, Anti-PKC98E antiserum localizes PKC98E (red) to the olfactory neuron dendrites (arrowhead) and a round structure between the base of the olfactory neuron dendrites and the cell body (arrow). E, Green is membrane-anchored tdGFP (Han et al., 2011) expressed under control of the ORCO promoter. Olfactory neuron dendrites and cell bodies are labeled. F, Co-localization of PKC98E and tdGFP in the olfactory neuron dendrites. G, Preimmune serum from the same rabbit later immunized against PKC98E peptide does not detect antigens in Drosophila antenna. H, PKC98E localization in w¹¹¹⁸ control. I, Flies expressing PKC98E RNAi with the ORCO promoter have reduced PKC98E signal. Scale bars: 10 μm (D) and 20 μm (G–I).