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. 2021 May 5;41(18):3948–3957. doi: 10.1523/JNEUROSCI.3019-20.2021

Figure 5.

Figure 5.

PKC98E conditional mutants are defective for ORCOS289 phosphorylation in vivo. A–D, Representative images showing the phosphorylation of ORCOS289 in the (A) w1118 control, (B) orco2 mutants that lack ORCO, (C) PKC98E FRT, and (D) PKC98E FRT conditional mutants (pORCO-GAL4; PKC98E FRT, UAS-FLP). Arrowheads point to representative olfactory neuron dendrites. E, The phosphorylation of ORCOS289 is strikingly reduced in PKC98E FRT conditional mutants (p = 0.009) and orco2 (p = 1.24 × 10−5) compared with wild-type controls (p = 0.009), but not in PKC98E FRT, UAS-FLP controls that lack the GAL4 driver (p = 0.41); n = 5–10, analysis by one-way ANOVA with post hoc Tukey's HSD test; ***p < 0.01 as compared with w1118 control. F–I, Representative images showing total ORCO localization in the control and conditional mutants. Arrowheads denote representative olfactory neuron dendrites. J, The quantification shows the overall ORCO localization in olfactory neuron dendrites is not significantly different among the genotypes except for the orco2 mutant that lacks ORCO (n = 15; p = 0.75 between wild-type and PKC98E FRT, UAS-FLP, and p = 0.59 between wild-type and PKC98E conditional, p = 2.77 × 10−6 between w1118 and orco2 mutant) by one-way ANOVA with post hoc Tukey's HSD test.