Table 1.
Structural parameters for the cryo-EM structures of fV and fVa
| Parameters | fV PDB ID:7KVE emd ID:23048 | fVa PDB ID:7KXY emd ID:23067 |
|---|---|---|
| Map | ||
| Voltage, kV | 300 | 300 |
| Electron exposure, e-/Å2 | 1.65 | 1.65 |
| Pixel size, Å | 1.1 | 1.1 |
| Initial particle images | 2631626 | 699611 |
| Final particle images | 299182 | 63745 |
| Symmetry imposed | C1 | C1 |
| Resolution, unmasked, FSC threshold 0.143, Å | 4.4 | 7.8 |
| Resolution, masked, FSC threshold 0.143, Å | 3.3 | 4.4 |
| Model | ||
| Refinement program | Phenix, real space | Phenix, real space |
| Number of protein atoms, non-H | 11142 | 9510 |
| RMSD bonds, Å | 0.005 | 0.004 |
| RMSD angles, ° | 0.816 | 1.069 |
| Ramachandran favored, % | 81.39 | 69.82 |
| Ramachandran allowed, % | 18.25 | 26.02 |
| Ramachandran disallowed, % | 0.36 | 4.16 |
| All-atom clash score | 17 | 19 |
| MolProbity score | 2.5 | 3.3 |
emd, electron microscopy data; FSC, forward scatter.