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. 2021 Jun 2;41(6):BSR20204250. doi: 10.1042/BSR20204250

Figure 5. Effects of TMAO on the M1 and M2 polarization of RAW264.7 cells.

Figure 5

(A and B) The expression levels of M1 (iNOS, TNF-α, IL-6 and IL-7) and M2 (Arg1, IL-10, MR and YM1) macrophagocyte markers in RAW264.7 cells treated with TMAO (0, 1, 3, 10, 30, 100 μM of 24 h) were determined by using RT-PCR. (C) The mRNA levels of Arg1, IL-10, MR and YM1 were detected by RT-PCR in RAW264.7 cells treated with IL-4 (20 ng/ml; 12 h) or IL-13 (10 ng/ml; 72 h) and TMAO (100 μM; 24 h; n=3, *P<0.05, compared with the control group; #P<0.05, compared with the IL-13 group; +P<0.05, compared with the IL-4 group).