Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;12(5):2720–2733. doi: 10.1364/BOE.414680

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

https://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Fig. 1. — Data processing pipeline for ML-SIM. Training data for the model is generated by taking images from the commonly used image dataset DIV2K and simulating the imaging process of SIM using a model adapted from the open source library OpenSIM. The simulation can be further optimised to reflect the properties of the experimental system for which the reconstruction method is desired, for example to match the pixel size of the detector or numerical aperture of the detection optics. The outputs of the simulation are image stacks of the same size as those acquired by the microscope (here 9 frames).