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. 2021 Apr 15;12(5):2720–2733. doi: 10.1364/BOE.414680

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

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Fig. 4. — Reconstruction of a SIM image of tubulin structures. The reconstruction output of ML-SIM is compared with a wide-field projected image and FairSIM. (Top) Full field-of-view of reconstructed image and line profiles across two parallel microtubules at the position indicated by the red line. While the microtubules are not resolved in widefield mode, both ML-SIM and FairSIM enable them to be clearly distinguished. (Bottom) Cropped regions of the reconstruction outputs corresponding to the area enclosed by the yellow rectangle.