Table 1.
Sequence of forward and reverse primers and TaqMan™ probes used for the SORP assay. Position of the Spike and ORF 8 primers and probes based on the alignment to the Wuhan-Hu-1 SARS-CoV-2 sequence (RefSeq:NC_045512.2).
| PRIMER/PROBE | SEQUENCE | Nucleotide Position | Ref |
|---|---|---|---|
| Spike-F1 | CCACTAGTCTCTAGTCAGTGTGTTAATY | 21568–21595 | Present work |
| Spike-R1 | AAACTGAGGATCTGAAAACTTTGTC | 21618–21647 | |
| Spike-P1 | FAM-CAACCAGAA/ZEN/CTCAATTACCCCCTGCATACA-IABlkFQ/ | 21690–21716 | |
| ORF8-F1 | GGAGCTAGAAAATCAGCACCTTTAA | 28041–28065 | Present work |
| ORF8-R | TCGATGTACTGAATGGGTGATTTAG | 28093–28117 | |
| ORF8-P | Cy5-TGAATTGTG/TAO/CSTGGATGAGGCTGG-IABlkRQ/ | 28067–28090 | |
| RNaseP-F | AGATTTGGACCTGCGAGCG | US-CDC | |
| RNaseP-P | NED-TTCTGACCTGAAGGCTC-MGBNFQ | BCCDC in house design (Lee) Modified from US-CDC for MGB | |
| RNaseP-R | GAGCGGCTGTCTCCACAAGT | US-CDC | |
| RNaseP-R8 | TCAACGATATGATTGATAGCAACAAC | Present work |