Table 2.
CT values obtained by individual RNAseP and mRNAseP assays, for two different template types-purified human gDNA and total nucleic acid (gDNA + mRNA). The TNA template was used with reverse transcription (+RT) and without reverse transcription (-RT).
| RNaseP TaqMan™ Assay | RT-qPCR Primer/Probes |
Amplicon Size |
TaqMan™ control Human gDNA (0.1 ng/μL) CT |
SARS-CoV-2 NEGATIVE Total Nucleic Acid (mRNA + gDNA) CT |
||||
|---|---|---|---|---|---|---|---|---|
| Forward | Probe | Reverse | mRNA | gDNA | +RT | -RT | ||
| RNaseP | RNaseP-F | RNase-P Probe | RNase-P-R | 65 bp | 65 bp | 32.88 | 27.25 | 28.3 |
| mRNaseP | RNaseP-F | RNase-P Probe | RNase-P-R8 | 105 bp | 2.8 kb | und | 30.14 | und |